Discuss how staining techniques may be applied in the identification of an unknown sample.

In this essay, you are asked to demonstrate your understanding of the different types of culturing and staining procedures discussed in Chapters 3 and 7. You should work on researching and composing this essay over the course of Modules 2 and 3.

Consider the following scenario:

You are given a sputum sample from a patient that may be infected with bacteria from one of the following genera:

Bacillus
Escherichia
Mycobacteria
For this essay,

Describe the handing of the specimen and how the infectious agent, in this case a bacterial pathogen, is isolated from the sputum sample.
Explain techniques used to isolate bacteria from a clinical specimen using the LearnSmart laboratory exercise, “Isolation Methods” as well as Chapter 7 to support your account.
Discuss how staining techniques may be applied in the identification of an unknown sample.
Discuss each staining protocol. Mention the steps involved and how each step and each protocol would identify or eliminate each of these genera as a suspect. You may refer to other scientific resources, but they should be in addition to and not in place of the module resources.
Describe the important anatomical differences among these three genera and the staining protocols you would use to identify which genus is causing the patient’s infection.


 

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